| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Salvi, Riley (8)
|
ADJJSC |
49.50 |
46.17 |
-3.33 |
| 2 |
Hopke, Lillian G (6)
|
ADCPP |
49.88 |
46.54 |
-3.34 |
| 3 |
Stewart Fraser, Thornton R (8)
|
ADALBS |
48.87 |
48.57 |
-0.30 |
| 4 |
Pacinella, Juliann F (8)
|
ADCPP |
48.30 |
49.72 |
1.42 |
| 5 |
Cho, Anna Y (8)
|
ADALBS |
50.23 |
49.99 |
-0.24 |
| 6 |
Cao, Aimee W (8)
|
ADJJSC |
49.69 |
50.54 |
0.85 |
| 7 |
Harpootlian, Leah C (7)
|
ADCPP |
57.29 |
52.16 |
-5.13 |
| 8 |
O'Donnell, Bridgett M (8)
|
ADEELS |
59.47 |
53.17 |
-6.30 |
| 9 |
Ramani, Akshaya K (8)
|
ADCPP |
59.10 |
54.03 |
-5.07 |
| 10 |
Yager, Myah Y (8)
|
ADCANA |
54.75 |
54.60 |
-0.15 |
| 11 |
Zhang, Serena Y (7)
|
ADCPP |
55.93 |
54.89 |
-1.04 |
| 12 |
Zhou, Yixuan (8)
|
ADCPP |
53.97 |
57.19 |
3.22 |
| 13 |
Hopke, Stella M (5)
|
ADCPP |
56.81 |
57.94 |
1.13 |
| 14 |
Banik, Noel Y (8)
|
ADALBS |
56.41 |
58.15 |
1.74 |
| 15 |
Ruff, Mikaella K (8)
|
ADALBS |
58.82 |
1:00.31 |
1.49 |
| 16 |
Childrose, Shea M (8)
|
ADJJSC |
57.07 |
1:00.53 |
3.46 |
| 17 |
Currier, Lillian G (7)
|
ADCPP |
57.97 |
1:01.35 |
3.38 |
| 18 |
Harpootlian, Emma L (6)
|
ADCPP |
1:13.15 |
1:07.15 |
-6.00 |
| 19 |
Huang, Grace X (8)
|
ADCPP |
1:01.12 |
1:09.53 |
8.41 |
| 20 |
Iyer, Snigdha P (7)
|
ADCPP |
1:13.31 |
1:20.30 |
6.99 |
|
Miller, Isabella M (8)
|
ADCPP |
1:04.25 |
DQ |
- |
|
Scapillato, Ella K (8)
|
ADCPP |
56.94 |
NS |
- |